TU_Flow_2

Paper: Whole Blood Fixation and Permeabilization Protocol with Red Blood Cell Lysis for Flow Cytometry of Intracellular Phosphorylated Epitopes in Leukocyte Subpopulations (Chow, Hedley et al., Cytometry Part A, 2005).

Paper: Whole Blood Processing for Measurement of Signaling Proteins by Flow Cytometry (Chow, Hedley and Shankey, Current Protocols in Cytometry, 2008).

Book: Practical Flow Cytometry, Howard Shapiro, 4th Edition, 2003. A comprehensive overview of flow cytometry.

Review article: Guidelines for the use of flow cytometry and cell sorting
in immunological studies (Cossarizza et al, European Journal of Immunology, 2019). A recent and comprehensive overview of flow cytometer (almost 500 pages).

Paper: Basic Multicolor Flow Cytometry (Maciorowski, Chattopadhyay and Jain, Current Protocols in Cytometry, 2017). A good introduction to high-dimensional flow cytometer.

Mario Roederer's Compensation Tutorial - a good basic introduction to fluorescence compensation - http://drmr.com/compensation/index.html

Paper: Compensation in Multicolor Flow Cytometry (Szaloki and Goda, Cytometry A, 2015). A good introduction to compensation.

Contacts:

Krishna Das Manandhar, Tribhuvan University, Kathmandu, NEPAL - krishna.manandhar@gmail.com
Zosia Maciorowski, Curie Institute, Paris, FRANCE - zosiamaciorowski@gmail.com
Derek Davies, Francis Crick Institute, London, UK - Derek.Davies@crick.ac.uk
Bill Telford, NCI-NIH, MD, USA - telfordw@mail.nih.gov
Paul Wallace, Roswell Park Cancer Institute, NY, USA - pkw@rpciflow.org
Awtar Krishan, University of Miami, FL, USA - akrishan@med.miami.edu
David Hedley, Pricess Margaret Hospital, Toronto, CANADA - david.hedley@uhn.ca
Hemant Agrawal, Flowcytometry Solutions Ltd., Jaipur, INDIA - flowsols@gmail.com
Lydia Tesfa, Cornell University, NY, USA - ltesfa917@gmail.com
H. Krishnamurthy, National Centre for Biological Sciences, Bangalore, INDIA - krishna@ncbs.res.in
Kylie Price, Malaghan Institute for Medical Research, Wellington, NEW ZEALAND - kprice@malaghan.org.nz

ISAC CYTO Virtual Interactive Conference 2021:

https://www.cytoconference.org

Website from 1st Nepal Virtual Flow Cytometry Workshop (February 2021):

https://www.cytometryunderground.com/TU_Flow_2021.html

CYTO University (CYTO U):

https://learning.isac-net.org/

OpenFlow Cytometry Video Lectures (Derek Davies, Francis Crick Institute)

https://www.youtube.com/channel/UCadgNlgcf6Zvu1HVGkvwp0w

Crick Webinars (Derek Davies, Francis Crick Institute)

https://www.youtube.com/playlist?list=PLpAyGqPO0aHrmHgwSV4oiU0M9G_j4wCXs