00:22:43 Krishna Manandhar: If any one from 00:23:25 William Telford: Workshop content at: https://www.cytometryunderground.com/TU_Flow_2021.html 00:24:25 Krishna Manandhar: If any one from undergraduate, Graduate, PhD, researcher and Faculties wish to say something about this 3-day workshop, please provide your name 00:32:20 Krishna Manandhar: Any one to share your experience at the end? 01:27:09 Gunnur Deniz: Hi Zosia 01:27:26 Gunnur Deniz: I am trying but at work it is difficult 01:27:33 Gunnur Deniz: Having another 01:27:45 Gunnur Deniz: Zoom meeting 01:27:45 Krishna Manandhar: Well come Prof. Gunner 01:28:10 Gunnur Deniz: Hi Krishna 01:28:38 Gunnur Deniz: Having a very useful course congratulations 01:36:00 Sharwan: Which one is better to perform compensation, while running the sample or before running the sample with dye only? 01:39:32 Zosia Maciorowski: You have to run your single color controls in order to calculate the compensation correctly. Usually it’s done first. on the Calibur , you can calculate and apply the compensation as you acquire the data. You can also calculate the compensation afterwards with a 3rd party software like FLowjo or FCS express, but you still need the single color control data to calculate 01:39:42 Purshotam Kumar Sah Kanu, CDMi (TU): How to set the compensation for our target sample? 01:41:20 Zosia Maciorowski: we will cover compensation in detail in the next session, but you can read the paper that Bill has uploaded on basics of Multicolour flow cytometry, it explains how. You always need a separate single color control for each color in the mix 01:42:25 Zosia Maciorowski: the compensation matrix calculated on your single color controls is then applied to your sample 01:42:34 Purshotam Kumar Sah Kanu, CDMi (TU): Thank You 01:58:43 Hemant Agrawal: http://drmr.com/compensation/index.html 01:59:24 Hemant Agrawal: https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.a.22736 02:09:38 Hemant Agrawal: https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.a.20549 02:09:56 Hemant Agrawal: paper about number of events to collect by Mario 02:12:05 Purshotam Kumar Sah Kanu, CDMi (TU): which one is the critical step in the flow cytometry either a cell sorting or compensation? 02:13:33 Zosia Maciorowski: The sample need to be correctly compensated, whether you are doing analysis only or sorting the cells. 02:14:22 Himal Chhetri: To which extent , Is it practicable for ploidy analysis of tumors? 02:15:05 William Telford: Yes you can do ploidy analysis using cell cycle analysis - labeling permeablized cells using a DNA dye like propidium iodide 02:15:44 Himal Chhetri: Thanks. 02:16:49 Zosia Maciorowski: We will do a session on ploidy and cell cycle in one of the future sessions 02:17:54 Ganesh Adhikari: Thank you for such a nice presentation but due to my network problem often I couldnot hear clearly so hoping to get recorded video too 02:18:38 Charu Arjyal: Thank you so much to the team! 02:18:44 eans tuladhar: thank you all for such an informative workshop 02:18:46 Ganga G.C: Thank you 02:18:55 NIRANJAN RANA: Thank you! 02:19:01 Soma Kanta Baral: Thank you 02:19:12 Suraj Chand (TU, CDBT): Thank you 02:19:38 Chandani karmacharya: thank you for such nice presentation 02:19:43 Dipendra Kumar Mandal: thank you all for informative workshop 02:20:14 Aruna Khanal: thank you so much for this wonderful workshop 02:20:17 Aashish Dhakal: Thank you Professors and Central Department of Biotechnology, TU for this wonderful opportunity. Looking forward to more workshop in the days ahead. 02:20:18 Pragati Pradhan: I would like to thank all the profesors for such a nice and fruitful presentation. 02:20:26 Rajan Bhandari (Msc.microbiology ;Trichandra): I feel very lucky to get chance to attend this workshop. I really got chance to enhance my level of knowledge and understanding on this topic. I am very impressed with the way of presentation and demonstration used here. Thank you so much professors and the organizer team. Hope i get chance to attend near future workshop on physical presence. 02:20:53 Gorakh Giri: Thank you organizer for very informative workshop 02:21:00 Suchitra Thapa: It was a very informative workshop. Congratulation to the organizing team. Thank you 02:21:15 Shova Shrestha: Thank you to the organizing committee & all the presenters for conducting such a wonderful workshop. 02:22:15 gajendra kumar das (m.sc. medical microbiology, trichandra clz): thank you team for providing this great opportunity. 02:23:16 Shova Shrestha: Congratulations Dr. Krishna Sir and the entire team for successfully conducting the program. 02:23:37 Pooja Shrestha: thank you isac team and central biotech department for organizing this event. it was indeed an insightful session. 02:23:54 Arun kumar Thakur: a big thanks to whole team for this workshop.. 02:24:30 Chetana Dahal CDMi TU: thank u for ur information 02:24:46 eans tuladhar: Thank you for providing many resource materials too, with this basic background,wish to attend the advanced learning too. 02:25:22 Deena Shrestha: Thank you for the workshop. It was very much informative and worthwhile. 02:25:43 Sofia Shahi: grateful towards the whole team for this wonderful and informative workshop.. thank you 02:42:15 Puja’s iPhone: good evening, greetings from Bhutan. it was a very informative sessions and hope to learn more in future as well. 02:43:06 William Telford: Thank you for visiting us from Bhutan! Maybe we can do a live workshop there someday! 02:45:05 Purshotam Kumar Sah Kanu, CDMi (TU): A well contribution from all side. Thanks to all of you. 02:45:32 Zosia Maciorowski: thank you, hopefully we will see you in Bhutan one day! 02:45:38 Khadga K.C.: director.cir@tu.edu.np or khadga.kc@dird.tu.edu.np